s aureus suspension  (ATCC)

Journal: Antibiotics

Article Title: β,β-Dimethylacrylalkannin Restores Colistin Efficacy Against mcr - and TCS-Mediated Resistant Gram-Negative Bacteria via Membrane Disturbance

doi: 10.3390/antibiotics15010003

Figure Lengend Snippet: β,β-Dim targets the phospholipids and disrupts cytoplasmic membrane biofunction. ( A ) Effects of β, β-Dim on membrane permeability in E. coli spheroplasts. ( B ) Intracellular ATP level in E. coli . ( C ) Influence of PE, PG, and CL on the antibacterial activity of β, β-Dim against S. aureus ATCC 29213. ( D ) Binding affinity of β, β-Dim for CL measured by ITC assays. ( E ) Intracellular β,β-Dim levels in E. coli BW25113, E. coli BW25113- mcr-1 , and E. coli BW25113- pmrB R93P following treatment with varying concentrations of colistin. Data represent three biological replicates. COL: colistin. Unit: μg/mL. ns, no significant difference. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The S. aureus ATCC 29213 bacterial suspension (OD600 = 0.5) was incubated with fluorescent probe propidium iodide (PI, Cat No. C1008S, Beyotime, Shanghai, China, final concentration 10 μM) for 30 min. Then, β,β-Dim (0–32 μg/mL) was added to the PI-labeled suspension in a black 96-well plate and incubated for 1 h. Fluorescence intensity was measured at an excitation/emission wavelength of 535/615 nm.

Techniques: Membrane, Permeability, Activity Assay, Binding Assay